Journal: Cell Cycle

Article Title: Direct conversion of mouse fibroblasts to GABAergic neurons with combined medium without the introduction of transcription factors or miRNAs

doi: 10.1080/15384101.2015.1060382

Figure Lengend Snippet: Characterization of MEFs. Immunostaing of the MEF cells, Vimentin and E-cadherin. Neural Markers: Nestin, Map2, Tuj-1, GFAP, Sox1,NeuN. scale bar, 50μm.

Article Snippet: Immunofluorescence staining Cells grown on coverslips were fixed with 4% paraformaldehyde (PFA, Sigma), blocked with 3% BSA, incubated overnight at 4°C with the primary antibodies as follows: rabbit anti-Vimentin (1:200, BD), mouse anti-E-cadherin (1:200, BD), mouse anti-Nestin (1:100, Cell Signaling Technology), Rabbit anti-Pax6 (1:50, Abcam), rabbit anti-GFAP (1:500, DAKO), mouse anti-Tuj1 (1:200, Sigma), rabbit anti-synapsin (1:200, Abcam), rabbit anti-GΑΒΑ (1:100, Abcam), mouse anti-p75 (1:200, Abcam), rabbit NeuN (1:100, Cell Signaling Technology), rabbit Map2 (1:100, Cell Signaling Technology), mouse Neurofilament-L (NF-L, 1:100, Cell Signaling Technology), mouse VGlut1 (1:100, millipore), chicken TH (1:200, GeneTex) and guinea pig GATA transporter 1 (GAT1,1:100, Synaptic System).

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